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Characterization of Citrus tristeza virus isolates from Colombia Rev. Protección Veg.
Morales,Juan; Orlando,Acosta; Tamayo,Pablo; Peñaranda,José.
Isolates of Citrus tristeza virus around the world differ widely in their biological properties. These properties may depend on strain mixtures within individual trees. Monoclonal Antibody 13 (MCA13) is an antibody that detects almost all the severe strains causing Quick Decline and Stem Pitting CTV syndromes. The objectives of this work were: I) to determine the percentage of CTV isolates reactive to the MCA13 antibody in each Colombian region evaluated and II) to analyze the genetic diversity of isolates from these regions. In almost all the regions evaluated, CTV MCA13 reactive isolates were identified; however, only one was found in Mompox area. The coat protein gene was amplified by reverse transcription-polymerase chain reaction (RT-PCR) and analyzed...
Tipo: Journal article Palavras-chave: Virus cross-protection; Citrus tristeza disease; Citrus spp; RT-PCR; SSCP.
Ano: 2013 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522013000100006
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